PTX3 ELISA Kit

Catalog No. ABIN1672820

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse PTX3.

Quick Overview for PTX3 ELISA Kit (ABIN1672820)

Target: PTX3 (Pentraxin 3 (PTX3))

Binding Specificity: AA 18-381

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (EDTA)

Product Details PTX3 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PTX3/Pentraxin 3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: E18-S381

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: E18-S381

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: mouse PTX3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for PTX3 has been precoated onto 96-well plates. Standards(NSO, E18-S381) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for PTX3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse PTX3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL mouse PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse PTX3 standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1274, Standard deviation: 56.1, CV(%): 4.4
• Sample 2: n=16, Mean(pg/ml): 2937, Standard deviation: 164.5, CV(%): 5.6
• Sample 3: n=16, Mean(pg/ml): 5436, Standard deviation: 342.5, CV(%): 6.3,
• Sample 1: n=24, Mean(pg/ml): 1435, Standard deviation: 83.23, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 3356, Standard deviation: 245, CV(%): 7.3
• Sample 3: n=24, Mean(pg/ml): 5628, Standard deviation: 382.7, CV(%): 6.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for PTX3

Target: PTX3 (Pentraxin 3 (PTX3))

Alternative Name: PTX3

Background:

Protein Function: Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self- components and female fertility. .

Background: PTX3(Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Synonyms: Pentraxin-related protein PTX3,Pentaxin-related protein PTX3,Tumor necrosis factor-inducible gene 14 protein,TSG-14,Ptx3,Tsg14,

Full Gene Name: Pentraxin-related protein PTX3

Cellular Localisation: Secreted.

Gene ID: 19288

UniProt: P48759